diachrome InVitro Study – UVM-2000

Presented at the 17^th International Diabetes Federation Congress

November 9, 2000 – Mexico City, Mexico

Chromium Picolinate And Biotin Enhance Glycogen Synthesis

And Glycogen Synthase Gene Expression In Human Skeletal Muscle Culture

zhong Q. Wang, Xian H. Zhang, and William T. Cefalu.  Department of Medicine, University of Vermont College of Medicine, Burlington, VT 05405

  Insulin resistance is characterized by a reduction in non-oxidative glucose disposal (i.e., glycogen synthesis) in insulin stimulated conditions.  Supplementation with nutrients such as chromium picolinate (CrPic) has shown favorable results on insulin action in vivo and, when CrPic is combined with biotin, an enhanced glucose uptake has been observed in tissue culture.  To evaluate the potential mechanism, we incubated human skeletal muscle culture (HSMC) with CrPic, biotin, and combination CrPic/Biotin. 

   HSMC cell line was plated and grown in 5% C0₂ in a specially formulated human skeletal muscle growth media.  Upon desired confluence (about 80%), CrPic (10 ng/ml), biotin (10 pmol), or CrPic (10 ng/ml) plus biotin (10 pmol) was added to the media.  Isovolumetric 0.9% NaCl was used as the control.  After incubation for 24 hr, cells were fasted in skeletal muscle basic media without 10% FCS for 2 hr.  Glycogen synthesis was analyzed under basal condition and after l00 nM insulin and 30 mM d-glucose were added and incubated for 2 hr.  Glycogen synthase (GS) mRNA was determined by RT-PCR; GS kinase-3 (GSK-3) by Western blot. 

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